Efficient live fluorescence imaging of intraflagellar transport in mammalian primary cilia.

ABSTRACT: Intraflagellar transport (IFT) is a motile process critical for building most cilia, including those of mammalian cells. Defects in IFT lead to short or missing cilia, and in animals can cause defects in development, for example, in hedgehog-mediated signaling, as well as disease symptoms such as polycystic kidney disease or retinal degeneration. Understanding how IFT works is thus a high priority in ciliary biology. Imaging of living cells has played a key role in understanding the mechanism of IFT and this is particularly the case in mammalian cells where biochemical analysis of IFT is extremely difficult due to the difficulty of isolating cilia away from the rest of the cell. Imaging IFT in living mammalian cells requires solution to several problems: constructing cell lines that expres

SUBMITTER: Ishikawa H 

PROVIDER: S-EPMC5028138 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature