Erasure and reestablishment of random allelic expression imbalance after epigenetic reprogramming.

ABSTRACT: Clonal level random allelic expression imbalance and random monoallelic expression provides cellular heterogeneity within tissues by modulating allelic dosage. Although such expression patterns have been observed in multiple cell types, little is known about when in development these stochastic allelic choices are made. We examine allelic expression patterns in human neural progenitor cells before and after epigenetic reprogramming to induced pluripotency, observing that loci previously characterized by random allelic expression imbalance (0.63% of expressed genes) are generally reset to a biallelic state in induced pluripotent stem cells (iPSCs). We subsequently neuralized the iPSCs and profiled isolated clonal neural stem cells, observing that significant random allelic expression imbala

SUBMITTER: Jeffries AR 

PROVIDER: S-EPMC5029458 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature