Oxygen additions in serial femtosecond crystallographic protein structures.

ABSTRACT: In principle, serial femtosecond crystallography (SFX) could yield data sets that are completely free of the effects caused by slow, radiation-induced chemical reactions, for example, oxygen additions, responsible for radiation damage. However, experimental evidence is presented here that SFX data sets obtained by techniques that expose different parts of the same specimen to single pulses of radiation do not have this property, even if the specimen in question is frozen. The diffraction image of each such crystal obtained with the first pulse of radiation is certain to represent the structure of a protein that has not been modified chemically, but all of the images obtained subsequently from the same crystal will represent structures that have been modified to a lesser or greater extent b

SUBMITTER: Wang J 

PROVIDER: S-EPMC5029527 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature