A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems.

ABSTRACT: Cell-free protein synthesis (CFPS) systems allow for robust protein expression with easy manipulation of conditions to improve protein yield and folding. Recent technological developments have significantly increased the productivity and reduced the operating costs of CFPS systems, such that they can compete with conventional in vivo protein production platforms, while also offering new routes for the discovery and production of biotherapeutics. As cell-free systems have evolved, productivity increases have commonly been obtained by addition of components to previously designed reaction mixtures without careful re-examination of the essentiality of reagents from previous generations. Here we present a systematic sensitivity analysis of the components in a conventional Escherichia coli CFPS

SUBMITTER: Cai Q 

PROVIDER: S-EPMC5029582 | biostudies-literature | 2015 May-Jun

REPOSITORIES: biostudies-literature