DNA promoter hypermethylation in nipple fluid: a potential tool for early breast cancer detection.

ABSTRACT:

Introduction

Nipple fluid aspiration provides direct non-invasive sampling of fluid from the mammary ductal system, where the majority of breast cancers originate. DNA promoter hypermethylation ("methylation") occurs early and at high frequency in breast carcinogenesis, bearing the potential as a biomarker for cancer detection at its earliest stages. We assessed methylation in nipple fluid from breasts of healthy women, of women with sporadic breast cancer and of their contralateral breasts. Our goal was to investigate whether nipple fluid can be used as a reliable methylation biomarker source.

Materials and methods

Methylation levels of 13 genes were analysed by quantitative multiplex-methylation specific PCR (QM-MSP) in nipple fluid samples from breasts of healthy women, a

SUBMITTER: de Groot JS 

PROVIDER: S-EPMC5029741 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature