Deviations in human gut microbiota: a novel diagnostic test for determining dysbiosis in patients with IBS or IBD.

ABSTRACT:

Background

Dysbiosis is associated with many diseases, including irritable bowel syndrome (IBS), inflammatory bowel diseases (IBD), obesity and diabetes. Potential clinical impact of imbalance in the intestinal microbiota suggests need for new standardised diagnostic methods to facilitate microbiome profiling.

Aim

To develop and validate a novel diagnostic test using faecal samples to profile the intestinal microbiota and identify and characterise dysbiosis.

Methods

Fifty-four DNA probes targeting ≥300 bacteria on different taxonomic levels were selected based on ability to distinguish between healthy controls and IBS patients in faecal samples. Overall, 165 healthy controls (normobiotic reference collection) were used to develop a dysbiosis model with a bacterial pro

SUBMITTER: Casen C 

PROVIDER: S-EPMC5029765 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature