Identification of Claudin 1 Transcript Variants in Human Invasive Breast Cancer.

ABSTRACT:

Background

The claudin 1 tight junction protein, solely responsible for the barrier function of epithelial cells, is frequently down regulated in invasive human breast cancer. The underlying mechanism is largely unknown, and no obvious mutations in the claudin 1 gene (CLDN1) have been identified to date in breast cancer. Since many genes have been shown to undergo deregulation through splicing and mis-splicing events in cancer, the current study was undertaken to investigate the occurrence of transcript variants for CLDN1 in human invasive breast cancer.

Methods

RT-PCR analysis of CLDN1 transcripts was conducted on RNA isolated from 12 human invasive breast tumors. The PCR products from each tumor were resolved by agarose gel electrophoresis, cloned and sequenced. Genomic DN

SUBMITTER: Blanchard AA 

PROVIDER: S-EPMC5029943 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature