Bacterial cell wall biogenesis is mediated by SEDS and PBP polymerase families functioning semi-autonomously.

ABSTRACT: Multi-protein complexes organized by cytoskeletal proteins are essential for cell wall biogenesis in most bacteria. Current models of the wall assembly mechanism assume that class A penicillin-binding proteins (aPBPs), the targets of penicillin-like drugs, function as the primary cell wall polymerases within these machineries. Here, we use an in vivo cell wall polymerase assay in Escherichia coli combined with measurements of the localization dynamics of synthesis proteins to investigate this hypothesis. We find that aPBP activity is not necessary for glycan polymerization by the cell elongation machinery, as is commonly believed. Instead, our results indicate that cell wall synthesis is mediated by two distinct polymerase systems, shape, elongation, division, sporulation (SEDS)-family pro

SUBMITTER: Cho H 

PROVIDER: S-EPMC5030067 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature