Double adrenocortical adenomas harboring independent KCNJ5 and PRKACA somatic mutations.

ABSTRACT:

Objective

Co-secretion of cortisol and aldosterone can be observed in adrenal adenomas. The aim of this study was to investigate the molecular characteristics of a co-existing aldosterone- and a cortisol-producing adenoma (CPA) in the same patient.

Design and methods

Two different adenomas within the same adrenal gland from a 49-year-old female patient with primary aldosteronism (PA) and Cushing's syndrome (CS) were studied. Multiple formalin-fixed paraffin-embedded tumor blocks were used for the analysis. Immunohistochemistry (IHC) was performed using a specific antibody against aldosterone synthase (CYP11B2). DNA and RNA were isolated separately from CYP11B2-positive and -negative tumor regions based on CYP11B2 IHC results.

Results

CYP11B2 IHC clearly demonstrated t

SUBMITTER: Nanba K 

PROVIDER: S-EPMC5030510 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature