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Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9.

ABSTRACT: Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes.

SUBMITTER: Iwata S 

PROVIDER: S-EPMC5030659 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9.

Iwata Satoru S   Yoshina Sawako S   Suehiro Yuji Y   Hori Sayaka S   Mitani Shohei S  

Scientific reports 20160921

Balancer chromosomes are convenient tools used to maintain lethal mutations in heterozygotes. We established a method for engineering new balancers in C. elegans by using the CRISPR/Cas9 system in a non-homologous end-joining mutant. Our studies will make it easier for researchers to maintain lethal mutations and should provide a path for the development of a system that generates rearrangements at specific sites of interest to model and analyse the mechanisms of action of genes. ...[more]

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