Characterisation of phosphorylated nucleotides by collisional and electron-based tandem mass spectrometry.

ABSTRACT:

Rationale

Tandem mass spectrometry of phosphorylated ions can often yield a limited number of product ions owing to the labile nature of phosphate groups. Developing techniques to improve dissociation for this type of ion has implications for the structural characterisation of many different phosphorylated ions, such as those from nucleotides, pharmaceutical compounds, peptides and polymers.

Methods

Solutions of adenosine monophosphate, diphosphate and triphosphate (AMP, ADP and ATP) were studied in a hybrid linear ion trap-Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. Precursor ions with an overall single positive charge, including protonated nucleotides or nucleotide cations containing one, two or three sodium atoms, were isolated for tandem mass spe

SUBMITTER: Ball AT 

PROVIDER: S-EPMC5031208 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature