N-glycosylation enables high lateral mobility of GPI-anchored proteins at a molecular crowding threshold.

ABSTRACT: The protein density in biological membranes can be extraordinarily high, but the impact of molecular crowding on the diffusion of membrane proteins has not been studied systematically in a natural system. The diversity of the membrane proteome of most cells may preclude systematic studies. African trypanosomes, however, feature a uniform surface coat that is dominated by a single type of variant surface glycoprotein (VSG). Here we study the density-dependence of the diffusion of different glycosylphosphatidylinositol-anchored VSG-types on living cells and in artificial membranes. Our results suggest that a specific molecular crowding threshold (MCT) limits diffusion and hence affects protein function. Obstacles in the form of heterologous proteins compromise the diffusion coefficient and t

SUBMITTER: Hartel AJ 

PROVIDER: S-EPMC5031801 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature