Adjuvant role of Pseudomonas flagellin for Acinetobacter baumannii biofilm associated protein.

ABSTRACT:

Aim

To study immunogenicity of Pseudomonas N terminal flagellin as an adjuvant for Acinetobacter baumannii (A. baumannii) biofilm associated protein (Bap).

Methods

The N terminal flagellin gene was amplified. The pET28a (+) and polymerase chain reaction products were digested with HindIII and EcoR I. The ligation of N terminal flagellin into pET28a (‏+) was performed using T4 DNA ligase and was then transformed into Escherichia coli BL21 (DE3) as a suitable expression host. pET28a (‏+) vector harboring a conserved region of Bap from our previous work was used. The recombinant proteins were expressed, analyzed by SDS-PAGE method and was purified by affinity chromatography with His-Tag residues followed by confirmation with western blotting. Mice were immunized with recombinan

SUBMITTER: Sefidi MD 

PROVIDER: S-EPMC5031927 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature