Directed Differentiation of Zebrafish Pluripotent Embryonic Cells to Functional Cardiomyocytes.

ABSTRACT: A cardiomyocyte differentiation in vitro system from zebrafish embryos remains to be established. Here, we have determined pluripotency window of zebrafish embryos by analyzing their gene-expression patterns of pluripotency factors together with markers of three germ layers, and have found that zebrafish undergoes a very narrow period of pluripotency maintenance from zygotic genome activation to a brief moment after oblong stage. Based on the pluripotency and a combination of appropriate conditions, we established a rapid and efficient method for cardiomyocyte generation in vitro from primary embryonic cells. The induced cardiomyocytes differentiated into functional and specific cardiomyocyte subtypes. Notably, these in vitro generated cardiomyocytes exhibited typical contractile kinetics

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC5032289 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature