Dinoflagellate Gene Structure and Intron Splice Sites in a Genomic Tandem Array.

ABSTRACT: Dinoflagellates are one of the last major lineages of eukaryotes for which little is known about genome structure and organization. We report here the sequence and gene structure of a clone isolated from a cosmid library which, to our knowledge, represents the largest contiguously sequenced, dinoflagellate genomic, tandem gene array. These data, combined with information from a large transcriptomic library, allowed a high level of confidence of every base pair call. This degree of confidence is not possible with PCR-based contigs. The sequence contains an intron-rich set of five highly expressed gene repeats arranged in tandem. One of the tandem repeat gene members contains an intron 26,372 bp long. This study characterizes a splice site consensus sequence for dinoflagellate introns. Two t

SUBMITTER: Mendez GS 

PROVIDER: S-EPMC5032977 | biostudies-literature | 2015 Sep-Oct

REPOSITORIES: biostudies-literature