Toward an Objective Diagnostic Test for Bacterial Cellulitis.

ABSTRACT:

Background

Prior studies repeatedly showed that cultures of skin lesions diagnosed as "cellulitis" are usually negative. However, lack of a gold standard for diagnosis (against which culture might be judged) and failure to assess the human immune response are important limitations of prior work. In this pilot study, we aimed to develop a criterion standard for research on bacterial cellulitis, to evaluate the sensitivity of procalcitonin for bacterial cellulitis, and to use gene expression analysis to find other candidate diagnostic markers.

Methods

We classified lesions via biopsies, 16s rRNA gene detection, culture, and histopathology. We quantified procalcitonin expression in blood. We also used Nanostring technology to quantify transcription of immunomodulators that may

SUBMITTER: Pallin DJ 

PROVIDER: S-EPMC5033594 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature