Multiparametric profiling of non-small-cell lung cancers reveals distinct immunophenotypes.

ABSTRACT: BACKGROUND. Immune checkpoint blockade improves survival in a subset of patients with non-small-cell lung cancer (NSCLC), but robust biomarkers that predict response to PD-1 pathway inhibitors are lacking. Furthermore, our understanding of the diversity of the NSCLC tumor immune microenvironment remains limited. METHODS. We performed comprehensive flow cytometric immunoprofiling on both tumor and immune cells from 51 NSCLCs and integrated this analysis with clinical and histopathologic characteristics, next-generation sequencing, mRNA expression, and PD-L1 immunohistochemistry (IHC). RESULTS. Cytometric profiling identified an immunologically "hot" cluster with abundant CD8⁺ T cells expressing high levels of PD-1 and TIM-3 and an immunologically "cold" clust

SUBMITTER: Lizotte PH 

PROVIDER: S-EPMC5033841 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature