Single-pulse enhanced coherent diffraction imaging of bacteria with an X-ray free-electron laser.

ABSTRACT: High-resolution imaging offers one of the most promising approaches for exploring and understanding the structure and function of biomaterials and biological systems. X-ray free-electron lasers (XFELs) combined with coherent diffraction imaging can theoretically provide high-resolution spatial information regarding biological materials using a single XFEL pulse. Currently, the application of this method suffers from the low scattering cross-section of biomaterials and X-ray damage to the sample. However, XFELs can provide pulses of such short duration that the data can be collected using the "diffract and destroy" approach before the effects of radiation damage on the data become significant. These experiments combine the use of enhanced coherent diffraction imaging with single-shot XFEL r

SUBMITTER: Fan J 

PROVIDER: S-EPMC5034275 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature