A novel affinity-based method for the isolation of highly purified extracellular vesicles.

ABSTRACT: Extracellular vesicles (EVs) such as exosomes and microvesicles serve as messengers of intercellular network, allowing exchange of cellular components between cells. EVs carry lipids, proteins, and RNAs derived from their producing cells, and have potential as biomarkers specific to cell types and even cellular states. However, conventional methods (such as ultracentrifugation or polymeric precipitation) for isolating EVs have disadvantages regarding purity and feasibility. Here, we have developed a novel method for EV purification by using Tim4 protein, which specifically binds the phosphatidylserine displayed on the surface of EVs. Because the binding is Ca²⁺-dependent, intact EVs can be easily released from Tim4 by adding Ca²⁺ chelators. Tim4 purification, which we

SUBMITTER: Nakai W 

PROVIDER: S-EPMC5034288 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature