Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis.

ABSTRACT:

Objective

DNA methylation may be a stable epigenetic contributor to defining fat cell lineage.

Methods

We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis.

Results

Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of

SUBMITTER: Lim YC 

PROVIDER: S-EPMC5034609 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature