At-line determining spore germination of Penicillium chrysogenum bioprocesses in complex media.

ABSTRACT: Spore inoculum quality in filamentous bioprocesses is a critical parameter associated with viable spore concentration (1) and spore germination (2). It influences pellet morphology and, consequently, process performance. The state-of-the-art method to measure viable spore concentration is tedious, associated with significant inherent bias, and not applicable in real-time. Therefore, it is not usable as process analytical technology (PAT). Spore germination has so far been monitored using image analysis, which is hampered by complex medium background often observed in filamentous bioprocesses. The method presented here is based on the combination of viability staining and large-particle flow cytometry which enables measurements in real-time and hence aims to be applicable as a PAT tool. It

SUBMITTER: Ehgartner D 

PROVIDER: S-EPMC5035658 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature