Parametric analysis of colony morphology of non-labelled live human pluripotent stem cells for cell quality control.

ABSTRACT: Given the difficulties inherent in maintaining human pluripotent stem cells (hPSCs) in a healthy state, hPSCs should be routinely characterized using several established standard criteria during expansion for research or therapeutic purposes. hPSC colony morphology is typically considered an important criterion, but it is not evaluated quantitatively. Thus, we designed an unbiased method to evaluate hPSC colony morphology. This method involves a combination of automated non-labelled live-cell imaging and the implementation of morphological colony analysis algorithms with multiple parameters. To validate the utility of the quantitative evaluation method, a parent cell line exhibiting typical embryonic stem cell (ESC)-like morphology and an aberrant hPSC subclone demonstrating unusual colony

SUBMITTER: Kato R 

PROVIDER: S-EPMC5036041 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature