Genomic Mining of Phylogenetically Informative Nuclear Markers in Bark and Ambrosia Beetles.

ABSTRACT: Deep level insect relationships are generally difficult to resolve, especially within taxa of the most diverse and species rich holometabolous orders. In beetles, the major diversity occurs in the Phytophaga, including charismatic groups such as leaf beetles, longhorn beetles and weevils. Bark and ambrosia beetles are wood boring weevils that contribute 12 percent of the diversity encountered in Curculionidae, one of the largest families of beetles with more than 50000 described species. Phylogenetic resolution in groups of Cretaceous age has proven particularly difficult and requires large quantity of data. In this study, we investigated 100 nuclear genes in order to select a number of markers with low evolutionary rates and high phylogenetic signal. A PCR screening using degenerate prime

SUBMITTER: Pistone D 

PROVIDER: S-EPMC5036811 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature