Intratumoral interferon-gamma increases chemokine production but fails to increase T cell infiltration of human melanoma metastases.

ABSTRACT:

Introduction

Optimal approaches to induce T cell infiltration of tumors are not known. Chemokines CXCL9, CXCL10, and CXCL11 support effector T cell recruitment and may be induced by IFN. This study tests the hypothesis that intratumoral administration of IFNγ will induce CXCL9-11 and will induce T cell recruitment and anti-tumor immune signatures in melanoma metastases.

Patients and methods

Nine eligible patients were immunized with a vaccine comprised of 12 class I MHC-restricted melanoma peptides and received IFNγ intratumorally. Effects on the tumor microenvironment were evaluated in sequential tumor biopsies. Adverse events (AEs) were recorded. T cell responses to vaccination were assessed in PBMC by IFNγ ELISPOT assay. Tumor biopsies were evaluated for immune cell infil

SUBMITTER: Mauldin IS 

PROVIDER: S-EPMC5037033 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature