An Automated Microwell Platform for Large-Scale Single Cell RNA-Seq.

ABSTRACT: Recent developments have enabled rapid, inexpensive RNA sequencing of thousands of individual cells from a single specimen, raising the possibility of unbiased and comprehensive expression profiling from complex tissues. Microwell arrays are a particularly attractive microfluidic platform for single cell analysis due to their scalability, cell capture efficiency, and compatibility with imaging. We report an automated microwell array platform for single cell RNA-Seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We evaluate the level of cross-contamination in our platform by both

SUBMITTER: Yuan J 

PROVIDER: S-EPMC5037380 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature