Identification of Reference Genes for RT-qPCR Data Normalization in Cannabis sativa Stem Tissues.

ABSTRACT: Gene expression profiling via quantitative real-time PCR is a robust technique widely used in the life sciences to compare gene expression patterns in, e.g., different tissues, growth conditions, or after specific treatments. In the field of plant science, real-time PCR is the gold standard to study the dynamics of gene expression and is used to validate the results generated with high throughput techniques, e.g., RNA-Seq. An accurate relative quantification of gene expression relies on the identification of appropriate reference genes, that need to be determined for each experimental set-up used and plant tissue studied. Here, we identify suitable reference genes for expression profiling in stems of textile hemp (Cannabis sativa L.), whose tissues (isolated bast fibres and core) are chara

SUBMITTER: Mangeot-Peter L 

PROVIDER: S-EPMC5037828 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature