Candida antarctica Lipase B Immobilized onto Chitin Conjugated with POSS^® Compounds: Useful Tool for Rapeseed Oil Conversion.

ABSTRACT: A new method is proposed for the production of a novel chitin-polyhedral oligomeric silsesquioxanes (POSS) enzyme support. Analysis by such techniques as X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy confirmed the effective functionalization of the chitin surface. The resulting hybrid carriers were used in the process of immobilization of the lipase type b from Candida antarctica (CALB). Fourier transform infrared spectroscopy (FTIR) confirmed the effective immobilization of the enzyme. The tests of the catalytic activity showed that the resulting support-biocatalyst systems remain hydrolytically active (retention of the hydrolytic activity up to 87% for the chitin + Methacryl POSS^® cage mixture (MPOSS) + CALB after 24 h of the immobilization), as well as

SUBMITTER: Zdarta J 

PROVIDER: S-EPMC5037846 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature