The Human Milk Oligosaccharide 2'-Fucosyllactose Quenches Campylobacter jejuni-Induced Inflammation in Human Epithelial Cells HEp-2 and HT-29 and in Mouse Intestinal Mucosa.

ABSTRACT:

Background

Campylobacter jejuni causes diarrhea worldwide; young children are most susceptible. Binding of virulent C. jejuni to the intestinal mucosa is inhibited ex vivo by α_1,2-fucosylated carbohydrate moieties, including human milk oligosaccharides (HMOSs).

Objective

The simplest α_1,2-fucosylated HMOS structure, 2'-fucosyllactose (2'-FL), can be predominant at ≤5 g/L milk. Although 2'-FL inhibits C. jejuni binding ex vivo and in vivo, the effects of 2'FL on the cell invasion central to C. jejuni pathogenesis have not been tested. Clinical isolates of C. jejuni infect humans, birds, and ferrets, limiting studies on its mammalian pathobiology.

Methods

Human epithelial cells HEp-2 and HT-29 infected with the virulent C. jejuni strain 81-176 huma

SUBMITTER: Yu ZT 

PROVIDER: S-EPMC5037868 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature