Divergent Transcriptional Responses to Physiological and Xenobiotic Stress in Giardia duodenalis.

ABSTRACT: Understanding how parasites respond to stress can help to identify essential biological processes. Giardia duodenalis is a parasitic protist that infects the human gastrointestinal tract and causes 200 to 300 million cases of diarrhea annually. Metronidazole, a major antigiardial drug, is thought to cause oxidative damage within the infective trophozoite form. However, treatment efficacy is suboptimal, due partly to metronidazole-resistant infections. To elucidate conserved and stress-specific responses, we calibrated sublethal metronidazole, hydrogen peroxide, and thermal stresses to exert approximately equal pressure on trophozoite growth and compared transcriptional responses after 24 h of exposure. We identified 252 genes that were differentially transcribed in response to all three st

SUBMITTER: Ansell BR 

PROVIDER: S-EPMC5038253 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature