A High-Throughput Approach To Identify Compounds That Impair Envelope Integrity in Escherichia coli.

ABSTRACT: The envelope of Gram-negative bacteria constitutes an impenetrable barrier to numerous classes of antimicrobials. This intrinsic resistance, coupled with acquired multidrug resistance, has drastically limited the treatment options against Gram-negative pathogens. The aim of the present study was to develop and validate an assay for identifying compounds that increase envelope permeability, thereby conferring antimicrobial susceptibility by weakening of the cell envelope barrier in Gram-negative bacteria. A high-throughput whole-cell screening platform was developed to measure Escherichia coli envelope permeability to a β-galactosidase chromogenic substrate. The signal produced by cytoplasmic β-galactosidase-dependent cleavage of the chromogenic substrate was used to determine the degree of

SUBMITTER: Baker KR 

PROVIDER: S-EPMC5038306 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature