Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring.

ABSTRACT:

Background

The speed of insulin absorption after subcutaneous delivery is highly variable. Incorrect assumptions about insulin pharmacokinetics compromise effective glycemic regulation. Our ultimate goal is to develop a system to monitor insulin levels in vivo continuously, allowing pharmacokinetic parameters to be calculated in real time. We hypothesize that a bead-based detection system can be run on a flow-through microfluidic platform to measure insulin in subcutaneous fluid sampled via microdialysis. As a first step in development, we focused on microsphere-based measurement of insulin.

Methods

Polystyrene microspheres coated with an anti-insulin monoclonal antibody were exposed to insulin-containing solutions, and after addition of a fluorescently labeled anti-insulin

SUBMITTER: Kahanovitz L 

PROVIDER: S-EPMC5038538 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature