Ontology highlight
ABSTRACT:
SUBMITTER: Lake BB
PROVIDER: S-EPMC5038589 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20160601 6293
The human brain has enormously complex cellular diversity and connectivities fundamental to our neural functions, yet difficulties in interrogating individual neurons has impeded understanding of the underlying transcriptional landscape. We developed a scalable approach to sequence and quantify RNA molecules in isolated neuronal nuclei from a postmortem brain, generating 3227 sets of single-neuron data from six distinct regions of the cerebral cortex. Using an iterative clustering and classifica ...[more]