Manipulation of IL-10 gene expression by Toxoplasma gondii and its products.

ABSTRACT:

Background

This study was designed to evaluate whether or not T. gondii and its derivatives can change the gene expression level of IL-10 in murine leukocytes in vivo.

Methods

Fifty BALB/c mice were divided into 5 groups, four of which received the excretory/secretory product (ESP) from cell culture medium, the ESP from cell free medium, the Toxoplasma lysate product (TLP) and the active tachyzoites, respectively. The fifth group was considered as control and received PBS. The peritoneal leukocytes from the mice were collected. Their total RNA were extracted and converted to cDNA and the gene expression levels of IL-10 in the samples were evaluated by quantitative real time-PCR using the REST-2009 software.

Results

The findings showed a decrease in the expression leve

SUBMITTER: Pestechian N 

PROVIDER: S-EPMC5038996 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature