Large-scale profiling of signalling pathways reveals an asthma specific signature in bronchial smooth muscle cells.

ABSTRACT:

Background

Bronchial smooth muscle (BSM) cells from asthmatic patients maintain in vitro a distinct hyper-reactive ("primed") phenotype, characterized by increased release of pro-inflammatory factors and mediators, as well as hyperplasia and/or hypertrophy. This "primed" phenotype helps to understand pathogenesis of asthma, as changes in BSM function are essential for manifestation of allergic and inflammatory responses and airway wall remodelling.

Objective

To identify signalling pathways in cultured primary BSMs of asthma patients and non-asthmatic subjects by genome wide profiling of differentially expressed mRNAs and activated intracellular signalling pathways (ISPs).

Methods

Transcriptome profiling by cap-analysis-of-gene-expression (CAGE), which permits selectio

SUBMITTER: Alexandrova E 

PROVIDER: S-EPMC5039037 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature