Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis.

ABSTRACT: The most important enzyme of the phenylpropanoid pathway, 4-coumarate:coenzyme A ligase (4CL), is encoded by several homologous genes including 4CL1. The 4CL1 promoter is a tissue-specific gene expression element, particularly active in the secondary xylem or older stems. In this study, the 1127 bp 5'- upstream region of the 4CL1 coding sequence from Eucalyptus camaldulensis, Euc4CL1, was isolated and characterized. Essential putative cis-elements in the Euc4CL1 promoter included: a TATA-box at -22/-28 position, two CCAAT-boxes at -256/-260 and -277/-281 positions, respectively, an AC-element at -328/-336 and A-boxes at -115/-120 and -990/-995 positions. To investigate the effect of the Euc4CL1 promoter on gene expression, a plant transformation ve

SUBMITTER: Hue HT 

PROVIDER: S-EPMC5039157 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature