Coexistence of multiple minor states of fatty acid binding protein and their functional relevance.

ABSTRACT: Proteins are dynamic over a wide range of timescales, but determining the number of distinct dynamic processes and identifying functionally relevant dynamics are still challenging. Here we present the study on human intestinal fatty acid binding protein (hIFABP) using a novel analysis of ¹⁵N relaxation dispersion (RD) and chemical shift saturation transfer (CEST) experiments. Through combined analysis of the two types of experiments, we found that hIFABP exists in a four-state equilibrium in which three minor states interconvert directly with the major state. According to conversion rates from the major "closed" state to minor states, these minor states are irrelevant to the function of fatty acid transport. Based on chemical shifts of the minor states which could not be determi

SUBMITTER: Yu B 

PROVIDER: S-EPMC5039767 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature