STRUM: structure-based prediction of protein stability changes upon single-point mutation.

ABSTRACT:

Motivation

Mutations in human genome are mainly through single nucleotide polymorphism, some of which can affect stability and function of proteins, causing human diseases. Several methods have been proposed to predict the effect of mutations on protein stability; but most require features from experimental structure. Given the fast progress in protein structure prediction, this work explores the possibility to improve the mutation-induced stability change prediction using low-resolution structure modeling.

Results

We developed a new method (STRUM) for predicting stability change caused by single-point mutations. Starting from wild-type sequences, 3D models are constructed by the iterative threading assembly refinement (I-TASSER) simulations, where physics- and knowledge-bas

SUBMITTER: Quan L 

PROVIDER: S-EPMC5039926 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature