Association of MTHFR (C677T) Gene Polymorphism With Breast Cancer in North India.

ABSTRACT:

Background

Breast cancer is one of the most common malignancies in women and is associated with a variety of risk factors. The functional single-nucleotide polymorphism (SNP) C677T in the gene encoding 5,10-methylenetetrahydrofolate reductase (MTHFR) may lead to decreased enzyme activity and affect the chemosensitivity of tumor cells. This study was designed to investigate the association of MTHFR gene polymorphism (SNP) in the pathogenesis of breast cancer among the North Indian women population.

Materials and methods

Genotyping was performed by polymerase chain reaction (PCR) using genomic DNA, extracted from the peripheral blood of subjects with (275 cases) or without (275 controls) breast cancer. Restriction fragment length polymorphism was used to study C6

SUBMITTER: Waseem M 

PROVIDER: S-EPMC5040218 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature