A Macroscopic Mathematical Model for Cell Migration Assays Using a Real-Time Cell Analysis.

ABSTRACT: Experiments of cell migration and chemotaxis assays have been classically performed in the so-called Boyden Chambers. A recent technology, xCELLigence Real Time Cell Analysis, is now allowing to monitor the cell migration in real time. This technology measures impedance changes caused by the gradual increase of electrode surface occupation by cells during the course of time and provide a Cell Index which is proportional to cellular morphology, spreading, ruffling and adhesion quality as well as cell number. In this paper we propose a macroscopic mathematical model, based on advection-reaction-diffusion partial differential equations, describing the cell migration assay using the real-time technology. We carried out numerical simulations to compare simulated model dynamics with data of obse

SUBMITTER: Di Costanzo E 

PROVIDER: S-EPMC5040252 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature