On-Chip Clonal Analysis of Glioma-Stem-Cell Motility and Therapy Resistance.

ABSTRACT: Enhanced glioma-stem-cell (GSC) motility and therapy resistance are considered to play key roles in tumor cell dissemination and recurrence. As such, a better understanding of the mechanisms by which these cells disseminate and withstand therapy could lead to more efficacious treatments. Here, we introduce a novel micro-/nanotechnology-enabled chip platform for performing live-cell interrogation of patient-derived GSCs with single-clone resolution. On-chip analysis revealed marked intertumoral differences (>10-fold) in single-clone motility profiles between two populations of GSCs, which correlated well with results from tumor-xenograft experiments and gene-expression analyses. Further chip-based examination of the more-aggressive GSC population revealed pronounced interclonal variations i

SUBMITTER: Gallego-Perez D 

PROVIDER: S-EPMC5040341 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature