A screening-based platform for the assessment of cellular respiration in Caenorhabditis elegans.

ABSTRACT: Mitochondrial dysfunction is at the core of many diseases ranging from inherited metabolic diseases to common conditions that are associated with aging. Although associations between aging and mitochondrial function have been identified using mammalian models, much of the mechanistic insight has emerged from Caenorhabditis elegans. Mitochondrial respiration is recognized as an indicator of mitochondrial health. The Seahorse XF96 respirometer represents the state-of-the-art platform for assessing respiration in cells, and we adapted the technique for applications involving C. elegans. Here we provide a detailed protocol to optimize and measure respiration in C. elegans with the XF96 respirometer, including the interpretation of parameters and results. The protocol takes ∼2 d to complete, ex

SUBMITTER: Koopman M 

PROVIDER: S-EPMC5040492 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature