A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing.

ABSTRACT:

Background

Understanding the pathogenic role of extracellular vesicles (EVs) in disease and their potential diagnostic and therapeutic utility is extremely reliant on in-depth quantification, measurement and identification of EV sub-populations. Quantification of EVs has presented several challenges, predominantly due to the small size of vesicles such as exosomes and the availability of various technologies to measure nanosized particles, each technology having its own limitations.

Materials and methods

A standardized methodology to measure the concentration of extracellular vesicles (EVs) has been developed and tested. The method is based on measuring the EV concentration as a function of a defined size range. Blood plasma EVs are isolated and purified using size exclusion

SUBMITTER: Vogel R 

PROVIDER: S-EPMC5040823 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature