Cacidases: caspases can cleave after aspartate, glutamate and phosphoserine residues.

ABSTRACT: Caspases are a family of proteases found in all metazoans, including a dozen in humans, that drive the terminal stages of apoptosis as well as other cellular remodeling and inflammatory events. Caspases are named because they are cysteine class enzymes shown to cleave after aspartate residues. In the past decade, we and others have developed unbiased proteomic methods that collectively identified ~2000 native proteins cleaved during apoptosis after the signature aspartate residues. Here, we explore non-aspartate cleavage events and identify 100s of substrates cleaved after glutamate in both human and murine apoptotic samples. The extended consensus sequence patterns are virtually identical for the aspartate and glutamate cleavage sites suggesting they are cleaved by the same caspases. Deta

SUBMITTER: Seaman JE 

PROVIDER: S-EPMC5041198 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature