Protective effect of 3-O-methyl quercetin and kaempferol from Semecarpus anacardium against H₂O₂ induced cytotoxicity in lung and liver cells.

ABSTRACT:

Background

Hydrogen peroxide is continuously generated in living cells through metabolic pathways and serves as a source of reactive oxygen species. Beyond the threshold level, it damages cells and causes several human disorders, including cancer.

Methods

Effect of isolated 3-O-methyl quercetin and kaempferol on H₂O₂ induced cytotoxicity, ROS formation, plasma membrane damage, loss of mitochondrial membrane potential, DNA damage was evaluated in normal liver and lung cells. The RT-PCR analysis used to determine Nrf 2 gene expression. Calorimetric ELISA was used to determine Nrf2 and p-38 levels. Expression of SOD and catalase was analyzed by Western blot analysis.

Results

The present study isolated 3-O-methyl quercetin and kaempferol from the ste

SUBMITTER: Kumar AD 

PROVIDER: S-EPMC5041319 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature