Three-dimensional immersive virtual reality for studying cellular compartments in 3D models from EM preparations of neural tissues.

ABSTRACT: Advances in the application of electron microscopy (EM) to serial imaging are opening doors to new ways of analyzing cellular structure. New and improved algorithms and workflows for manual and semiautomated segmentation allow us to observe the spatial arrangement of the smallest cellular features with unprecedented detail in full three-dimensions. From larger samples, higher complexity models can be generated; however, they pose new challenges to data management and analysis. Here we review some currently available solutions and present our approach in detail. We use the fully immersive virtual reality (VR) environment CAVE (cave automatic virtual environment), a room in which we are able to project a cellular reconstruction and visualize in 3D, to step into a world created with Blender,

SUBMITTER: Cali C 

PROVIDER: S-EPMC5042088 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature