Human Milk MicroRNA and Total RNA Differ Depending on Milk Fractionation.

ABSTRACT: MicroRNA have been recently discovered in human milk signifying potentially important functions for both the lactating breast and the infant. Whilst human milk microRNA have started to be explored, little data exist on the evaluation of sample processing, and analysis to ensure that a full spectrum of microRNA can be obtained. Human milk comprises three main fractions: cells, skim milk, and lipids. Typically, the skim milk fraction has been measured in isolation despite evidence that the lipid fraction may contain more microRNA. This study aimed to standardize isolation of microRNA and total RNA from all three fractions of human milk to determine the most appropriate sampling and analysis procedure for future studies. Three different methods from eight commercially available kits were test

SUBMITTER: Alsaweed M 

PROVIDER: S-EPMC5042114 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature