Determining the chemical exchange saturation transfer (CEST) behavior of citrate and spermine under in vivo conditions.

ABSTRACT:

Purpose

To estimate the exchange rates of labile (1) H in citrate and spermine, metabolites present in prostatic secretions, to predict the size of the citrate and spermine CEST effects in vivo.

Methods

CEST z-spectra were acquired at high-field [11.7 Tesla (T)] from citrate and spermine solutions at physiological pH (6.5) using saturation power 6 μT. CEST was performed at different temperatures to determine exchange regimes (slow, intermediate or fast). For low pH solutions of spermine, exchange rates were estimated from resonance line width, fitting z-spectra using the Bloch equations incorporating exchange, and using quantifying exchange using saturation time experiments (QUEST). These rates were extrapolated to physiological pH.

Results

Citrate showed little CEST

SUBMITTER: Basharat M 

PROVIDER: S-EPMC5042183 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature