Long Term Ex Vivo Culture and Live Imaging of Drosophila Larval Imaginal Discs.

ABSTRACT: Continuous imaging of live tissues provides clear temporal sequence of biological events. The Drosophila imaginal discs have been popular experimental subjects for the study of a wide variety of biological phenomena, but long term culture that allows normal development has not been satisfactory. Here we report a culture method that can sustain normal development for 18 hours and allows live imaging. The method is validated in multiple discs and for cell proliferation, differentiation and migration. However, it does not support disc growth and cannot support cell proliferation for more than 7 to 12 hr. We monitored the cellular behavior of retinal basal glia in the developing eye disc and found that distinct glia type has distinct properties of proliferation and migration. The live imaging

SUBMITTER: Tsao CK 

PROVIDER: S-EPMC5042436 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature