A hyperspectral method to assay the microphysiological fates of nanomaterials in histological samples.

ABSTRACT: Nanoparticles are used extensively as biomedical imaging probes and potential therapeutic agents. As new particles are developed and tested in vivo, it is critical to characterize their biodistribution profiles. We demonstrate a new method that uses adaptive algorithms for the analysis of hyperspectral dark-field images to study the interactions between tissues and administered nanoparticles. This non-destructive technique quantitatively identifies particles in ex vivo tissue sections and enables detailed observations of accumulation patterns arising from organ-specific clearance mechanisms, particle size, and the molecular specificity of nanoparticle surface coatings. Unlike nanoparticle uptake studies with electron microscopy, this method is tractable for imaging large fiel

SUBMITTER: SoRelle ED 

PROVIDER: S-EPMC5042654 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature