Blockade of Orai1 Store-Operated Calcium Entry Protects against Renal Fibrosis.

ABSTRACT: Evidence supports an important role of Ca²⁺ release-activated Ca²⁺ channel protein 1 (Orai1)-mediated Ca²⁺ entry in the development of renal fibrosis, a common pathologic feature of CKDs that lead to ESRD, but the molecular mechanisms remain unclear. We determined the role of Orai1 calcium channel in renal fibrosis induced by high-fat diet and by unilateral ureteral obstruction. Mouse kidneys with fibrosis had higher levels of Orai1 protein expression than did kidneys without fibrosis. In vivo knockdown of Orai1 with adenovirus harboring Orai1-short hairpin RNA or inhibition of Orai1 with SKF96365 dramatically prevented renal fibrosis and significantly decreased protein expression of fibronectin, α‑smooth muscle actin, and TGF‑β1 in the kidney cortex of Apo

SUBMITTER: Mai X 

PROVIDER: S-EPMC5042666 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature